聚酮类化合物(polyketide,PK)和非核糖多肽(nonribosonmal peptide,NRP)是两大类重要的微生物次级代谢产物,其生物合成需要聚酮合成酶(polyketide synthases, PKSs)和非核糖体多肽合成酶(non-ribosomal peptide synthetases, NRPSs)。随着测序技术和生物信息分析手段的快速发展, PKS/NRPS基因簇及其合成产物的信息飞速增长。整合资源,创建PKS/NRPS数据库,以方便科学家进行序列分析,产物结构预测,以及实现分子改造合成新型化合物等的研究。笔者主要介绍近5年开发的与PKS/NRPS相关的重要的数据库,包括PKMiner,NRPSsp,NaPDoS, ClusterMine360和IMG-ABC,为相关领域的研究人员提供参考。
Abstract
Polyketide synthases (PKSs) and nonribosomal peptide synthetases (NRPSs) are two kinds of multi-modular enzymes, which biosynthesize highly complex polyketides and nonribosonmal peptides, respectively. Both of these two secondary metabolites are of considerable pharmaceutical relevance and are thought to cover diverse biological functions. With the development of sequencing and bioinformatics, data about PKS/NRPS are increasing rapidly. New PKS/NRPS databases are created to analyze gene sequence and predict the functions and structures of natural products. In this article, we introduce five newest databases including PKMiner, NRPSsp, NaPDoS, ClusterMine360, and IMG-ABC, with the goal to help researchers choose databases.
关键词
聚酮合成酶 /
非核糖体多肽合成酶 /
数据库 /
序列分析 /
结构预测
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Key words
PKS /
NRPS /
databases /
sequence analysis /
structure prediction
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中图分类号:
R956
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脚注
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基金
国家自然科学基金资助项目(30973681); 浙江省博士后择优资助项目(BSH1502144);浙江工业大学自然科学研究基金资助项目(2014XY017);浙江工业大学药学院华海创新计划SIPH;浙江工业大学运河杯大学生课外学术科技基金资助项目(2015YH0528)
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